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Bound human IgG1 variants were detected by a goat anti-human IgG Fc specific AP-conjugated antibody (Sigma-Aldrich A5944) (diluted 1:5000 in PBS), and binding visualized by addition of AP-substrate (10 g/mL in diethanolamine buffer) (Sigma-Aldrich)

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A 2023 study published in Cell Reports added another layer

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